Ontology highlight
ABSTRACT:
SUBMITTER: Park MS
PROVIDER: S-EPMC6707842 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Molecular cell 20190716 4
Despite the relevance of Argonaute proteins in RNA silencing, little is known about the structural steps of small RNA loading to form RNA-induced silencing complexes (RISCs). We report the 1.9 Å crystal structure of human Argonaute4 with guide RNA. Comparison with the previously determined apo structure of Neurospora crassa QDE2 revealed that the PIWI domain has two subdomains. Binding of guide RNA fastens the subdomains, thereby rearranging the active-site residues and increasing the affinity f ...[more]