Loss of mcr Genes Mediated by Plasmid Elimination and ISApl1.
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ABSTRACT:
SUBMITTER: Zhang P
PROVIDER: S-EPMC6709468 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Publications
Loss of <i>mcr</i> Genes Mediated by Plasmid Elimination and IS<i>Apl1</i>.
Zhang Pei P Bai Li L Li Yan Y Wang Zhiqiang Z Li Ruichao R
Antimicrobial agents and chemotherapy 20190823 9
We characterized the stability of a plasmid pCP53-mcr1_3 encoding <i>mcr-1</i> and <i>mcr-3.19</i> with and without colistin exposure during cultural passages via S1-pulsed-field gel electrophoresis (PFGE) and nanopore MinION sequencing. Both <i>mcr-1</i> and <i>mcr-3.19</i> were missing in certain subclones, mediated by genetic excision (IS<i>Apl1-mcr-1-pap2</i>), and deletions of large multidrug resistance (MDR) regions confirmed by IS<i>Apl1</i> and plasmid elimination. Without colistin expos ...[more]
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