Dataset Information

Recombinant mussel protein Pvfp-5?: A potential tissue bioadhesive.

ABSTRACT: During their lifecycle, many marine organisms rely on natural adhesives to attach to wet surfaces for movement and self-defense in aqueous tidal environments. Adhesive proteins from mussels are biocompatible and elicit only minimal immune responses in humans. Therefore these proteins have received increased attention for their potential applications in medicine, biomaterials, and biotechnology. The Asian green mussel Perna viridis secretes several byssal plaque proteins, molecules that help anchoring the mussel to surfaces. Among these proteins, protein-5? (Pvfp-5?) initiates interactions with the substrate, displacing interfacial water molecules before binding to the surface. Here, we established the first recombinant expression in Escherichia coli of Pvfp-5?. We characterized recombinant Pvfp-5?, finding that despite displaying a CD spectrum consistent with features of a random coil, the protein is correctly folded as indicated by MS and NMR analyses. Pvfp-5? folds as a ?-sheet-rich protein as expected for an epidermal growth factor-like module. We examined the effects of Pvfp-5? on cell viability and adhesion capacity in NIH-3T3 and HeLa cell lines, revealing that Pvfp-5? has no cytotoxic effects at the protein concentrations used and provides good cell-adhesion strength on both glass and plastic plates. Our findings suggest that the adhesive properties of recombinant Pvfp-5? make it an efficient surface-coating material, potentially suitable for biomedical applications including regeneration of damaged tissues.

SUBMITTER: Santonocito R

PROVIDER: S-EPMC6709630 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Publications

Recombinant mussel protein Pvfp-5β: A potential tissue bioadhesive.

Santonocito Radha R Venturella Francesca F Dal Piaz Fabrizio F Morando Maria Agnese MA Provenzano Alessia A Rao Estella E Costa Maria Assunta MA Bulone Donatella D San Biagio Pier Luigi PL Giacomazza Daniela D Sicorello Alessandro A Alfano Caterina C Passantino Rosa R Pastore Annalisa A

The Journal of biological chemistry 20190710 34

During their lifecycle, many marine organisms rely on natural adhesives to attach to wet surfaces for movement and self-defense in aqueous tidal environments. Adhesive proteins from mussels are biocompatible and elicit only minimal immune responses in humans. Therefore these proteins have received increased attention for their potential applications in medicine, biomaterials, and biotechnology. The Asian green mussel <i>Perna viridis</i> secretes several byssal plaque proteins, molecules that he ...[more]

PMID: 31292195

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Project description:The combination of multiple physiological (swelling, porosity, mechanical, and biodegradation) and biological (cell/tissue-adhesive, cell proliferation, and hemostatic) properties on a single hydrogel has great potential for skin tissue engineering. Adhesive hydrogels based on polydopamine (PDA) have become the most popular in the biomedical field; however, integrating multiple properties on a single adhesive hydrogel remains a challenge. Here, inspired by the chemistry of mussels, we developed PDA-sodium alginate-polyacrylamide (PDA-SA-PAM)-based hydrogels with multiple physiological and biological properties for skin tissue engineering applications. The hydrogels were prepared by alkali-induced polymerization of DA followed by complexation with SA in PAM networks. The chemical composition of the hydrogels was characterized by X-ray photoelectron spectroscopy. PDA-SA complexed chains were homogeneously dispersed in the PAM network and exhibited good elasticity and excellent mechanical properties, such as a compressive stress of 0.24 MPa at a compression strain of 70% for 0.4PDA-SA-PAM. The adhesive hydrogel also maintained a highly interconnected porous structure (∼94% porosity) along with PDA microfibrils. The hydrogel possesses outstanding swelling and biodegradability properties. Owing to the presence of the PDA-SA complex in the PAM network, the hydrogels show good adhesion to various substrates (plastic, skin, glass, computer screens, and leaves); for example, the adhesive strength of the 0.4PDA-SA-PAM to porcine skin was 24.5 kPa. The adhesive component of the PDA-SA chains in the PAM network significantly improves the cell proliferation, cell attachment, cell spreading, and functional expression of human skin fibroblasts (CCD-986sk) and keratinocytes. Moreover, the PDA chains exhibited good hemostatic properties, resulting in rapid blood coagulation. Considering their excellent cell affinity, and rapid blood coagulation ability, these mussel-inspired hydrogels have substantial potential for skin tissue engineering applications.

Dataset Information

Recombinant mussel protein Pvfp-5?: A potential tissue bioadhesive.

Publications

Recombinant mussel protein Pvfp-5β: A potential tissue bioadhesive.

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