Human Intestinal Enteroids Model MHC-II in the Gut Epithelium.
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ABSTRACT:
SUBMITTER: Wosen JE
PROVIDER: S-EPMC6710476 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Publications
Human Intestinal Enteroids Model MHC-II in the Gut Epithelium.
Wosen Jonathan E JE Ilstad-Minnihan Alexandra A Co Julia Y JY Jiang Wei W Mukhopadhyay Dhriti D Fernandez-Becker Nielsen Q NQ Kuo Calvin J CJ Amieva Manuel R MR Mellins Elizabeth D ED
Frontiers in immunology 20190820
The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, "mini-guts" derived from small intestinal crypt stem cells, and show that the intracellular MHC-II peptide-pathway is intact and functional in IECs. Our approach enables human enteroids to be used for novel <i>in vitro</i> studies into IEC MHC-II regulation and function during health and disease. ...[more]
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