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Differential expression of circRNAs during rat lung development.

ABSTRACT: At present, thousands of circular RNAs (circRNAs) have been found in cancer and various tissues from different species. However, the expression of circRNAs during rat lung development remains largely unknown. In the present study, circRNA expression profiles were screened in three mixed rat lung tissues at 3 time?points [embryonic day (E) 19, E21 and post?natal (P) day 3] during fetal rat development with circRNA high?throughput sequencing. Preliminary results were verified by reverse transcription?PCR (RT?PCR) at 4 time?points (E16, E19, E21 and P3). A total of 375 circRNAs were differently expressed in E19 vs. E21 (fold change ?1.5; P<0.05). At the same time, a total of 358 circRNAs were differently expressed in E21 vs. P3 (fold change ?1.5; P<0.05). A total of 3 circRNAs (rno_circ:chr7:24777879?24784993, rno_circ:chr14:14620910?14624933 and rno_circ:chr3:1988750??1998592) were characterized by having consistent fold changes (?1.5) between 3 time?points (E19, E21 and P3) and were selected for RT?PCR at 4 time?points (E16, E19, E21 and P3). Subsequently, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis of parent genes of the differentially expressed circRNAs revealed that these circRNAs may serve important roles in lung development. The present results support that these new found circRNAs participate in lung development. Furthermore, these findings may help to clarify the physiopathological mechanism of normal rat lung development, and may further provide a physiopathological basis of lung developmental diseases.

SUBMITTER: Shen YQ

PROVIDER: S-EPMC6713411 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Differential expression of circRNAs during rat lung development.

Shen Yan-Qing YQ Pan Jing-Jing JJ Sun Zhong-Yi ZY Chen Xiao-Qing XQ Zhou Xiao-Guang XG Zhou Xiao-Yu XY Cheng Rui R Yang Yang Y

International journal of molecular medicine 20190802 4

At present, thousands of circular RNAs (circRNAs) have been found in cancer and various tissues from different species. However, the expression of circRNAs during rat lung development remains largely unknown. In the present study, circRNA expression profiles were screened in three mixed rat lung tissues at 3 time‑points [embryonic day (E) 19, E21 and post‑natal (P) day 3] during fetal rat development with circRNA high‑throughput sequencing. Preliminary results were verified by reverse transcript ...[more]

PMID: 31432143

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Project description:BackgroundRat liver regeneration (LR) proceeds along a process of highly organized and ordered tissue growth in response to the loss or injury of liver tissue, during which many physiological processes may play important roles. The molecular mechanism of hepatocyte proliferation, energy metabolism and substance metabolism during rat LR had been elucidated. Further, the correlation of circular RNA (circRNA) abundance with proliferation has recently been clarified. However, the regulatory capacity of circRNA in rat LR remains a fascinating topic.ResultsTo investigate the regulatory mechanism of circRNA during priming phase of rat LR, high-throughput RNA sequencing technology was performed to unbiasedly profile the expression of circRNA during priming phase of rat LR. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathway analysis was conducted to predict the functions of differentially expressed circRNAs and their host linear transcripts. Co-expression networks of circRNA-miRNA were constructed based on the correlation analysis between the differentially expressed LR-related circRNAs and the condition of their miRNA binding sites. To excavate the key circRNAs in the early phase of rat LR, we comprehensively evaluated and integrated the relationship of expression level between the circRNAs and the linear transcripts as well as the distribution of miRNA binding sites in circRNA sequences.ConclusionsThis paper is the first to employ the comprehensive circRNA expression profile and to investigate circRNA-miRNA interactions during priming phase of rat LR. Two thousand four hundred twelve circRNAs were detected, and 159 circRNAs deriving from 116 host linear transcripts differentially expressed (p < 0.05). Six significantly changed circRNAs during priming phase of rat LR were screened as key circle molecules, and then were validated by qRT-PCR. This study will lay the foundation for revealing the functional roles of circRNAs during rat LR and help solve the remaining clinical problems.

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Differential expression of circRNAs during rat lung development.

Publications

Differential expression of circRNAs during rat lung development.

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