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A magnetic affinity approach to identify plant GABA-binding proteins.

ABSTRACT: In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl) can obtain useful information on GABA receptors in plants.

SUBMITTER: Zou J 

PROVIDER: S-EPMC6713914 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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A magnetic affinity approach to identify plant GABA-binding proteins.

Zou Jie J   Guo Jingzhe J   Li Shisheng S  

Turkish journal of biology = Turk biyoloji dergisi 20190805 4

In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on  ...[more]

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