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One-Step Fabrication of Enzyme-Immobilized Reusable Polymerized Microcapsules from Microfluidic Droplets.

ABSTRACT: Enzyme immobilization is an essential prerequisite for biocatalysis. In this context, emulsion provides an excellent template for assembling enzymes at the oil-water interface. A microfluidic approach has been adopted to produce oil-in-water-type emulsions stabilized by gold nanoparticle-catalase conjugates. In situ ring-opening polymerization of the oil phase produces solid core enzyme-immobilized microcapsules (MCs). These resultant MCs exhibited a K m value of 42 mM and shows 1.1-fold higher activity compared to free enzymes. Finally, the robust MCs showed excellent recyclability, which can meet the demand of industrial biotechnological applications.

SUBMITTER: Varshney R

PROVIDER: S-EPMC6714293 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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One-Step Fabrication of Enzyme-Immobilized Reusable Polymerized Microcapsules from Microfluidic Droplets.

Varshney Rohit R Sharma Shubhra S Prakash Bhanu B Laha Joydev K JK Patra Debabrata D

ACS omega 20190812 9

Enzyme immobilization is an essential prerequisite for biocatalysis. In this context, emulsion provides an excellent template for assembling enzymes at the oil-water interface. A microfluidic approach has been adopted to produce oil-in-water-type emulsions stabilized by gold nanoparticle-catalase conjugates. In situ ring-opening polymerization of the oil phase produces solid core enzyme-immobilized microcapsules (MCs). These resultant MCs exhibited a <i>K</i> <sub>m</sub> value of 42 mM and show ...[more]

PMID: 31497697

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