Dataset Information

Targeted Infarction of the Internal Capsule in the Rat Using Microstimulation Guidance.

ABSTRACT: Background and Purpose- Lacunar strokes are subcortical infarcts with small size and high disability rates, largely due to injury of the corticospinal tract in the internal capsule (IC). Current rodent models of lacunar infarcts are created based on stereotactic coordinates. We tested the hypothesis that better understanding of the somatotopy of the IC and guiding the lesion with electrical stimulation would allow a more accurate lesion to the forelimb axons of the IC. Methods- We performed electrophysiological motor mapping and viral tracing to define the somatotopy of the IC of Sprague Dawley rats. For the lesion, we used an optrode, which contains an electrode to localize forelimb responses and an optical fiber to deliver light. The infarct was induced when light activated the photothrombotic agent Rose Bengal, which was administered systemically. Results- We found largely a separate distribution of the forelimb and hindlimb axons in the IC, both by microstimulation mapping and tract tracing. Microstimulation-guided IC lesions ablated the forelimb axons of the IC in rats and caused lasting forelimb impairments while largely preserving the hindlimb axons of the IC and surrounding gray matter. Conclusions- Stimulation guidance enabled selective and reproducible infarcts of the forelimb axons of the IC in rats. Visual Overview- An online visual overview is available for this article.

SUBMITTER: Wen TC

PROVIDER: S-EPMC6714571 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Targeted Infarction of the Internal Capsule in the Rat Using Microstimulation Guidance.

Wen Tong-Chun TC Sindhurakar Anil A Ramirez Violeta Contreras VC Park HongGeun H Gupta Disha D Carmel Jason B JB

Stroke 20190808 9

Background and Purpose- Lacunar strokes are subcortical infarcts with small size and high disability rates, largely due to injury of the corticospinal tract in the internal capsule (IC). Current rodent models of lacunar infarcts are created based on stereotactic coordinates. We tested the hypothesis that better understanding of the somatotopy of the IC and guiding the lesion with electrical stimulation would allow a more accurate lesion to the forelimb axons of the IC. Methods- We performed elec ...[more]

PMID: 31390970

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Dataset Information

Targeted Infarction of the Internal Capsule in the Rat Using Microstimulation Guidance.

Publications

Targeted Infarction of the Internal Capsule in the Rat Using Microstimulation Guidance.

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