Ontology highlight
ABSTRACT:
SUBMITTER: Wang Y
PROVIDER: S-EPMC6715627 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Wang Yong Y Zhang Kaining K Li Hanfei H Xu Xin X Xue Huijun H Wang Pingping P Fu Yu V YV
Scientific reports 20190829 1
Tuned gene expression is crucial to the proper growth and response to the environmental changes of an organism. To enable tunable gene expression as designed is desirable in both scientific research and industrial application. Here, we introduce a novel promoter switching method based on the DDI2 promoter (P<sub>DDI2</sub>) that can fine tune the expression of target genes. We constructed a recyclable cassette (P<sub>DDI2</sub>-URA3-P<sub>DDI2</sub>) and integrated it upstream of yeast target ge ...[more]