Dataset Information

Whole-Genome Sequencing of Six Strains of Salmonella enterica Isolated from Imported Meat in Algeria.

ABSTRACT: Nontyphoidal Salmonella (NTS) is one of the main causes of foodborne disease worldwide. In this report, we announce the first whole-genome sequencing of six strains of Salmonella enterica isolated from imported meat in Algeria. The genome sizes ranged from 4,601,209 to 4,958,962?bp. Antimicrobial resistance (AMR) genes, plasmids, and virulence factors were detected.

SUBMITTER: Deriet A

PROVIDER: S-EPMC6715865 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Whole-Genome Sequencing of Six Strains of Salmonella enterica Isolated from Imported Meat in Algeria.

Deriet A A Berrazeg M M De Keersmaecker S C J SCJ Botteldoorn N N Vanneste K K Verhaegen B B Roosens N H C NHC Mouffok F F Drali R R

Microbiology resource announcements 20190829 35

Nontyphoidal <i>Salmonella</i> (NTS) is one of the main causes of foodborne disease worldwide. In this report, we announce the first whole-genome sequencing of six strains of <i>Salmonella enterica</i> isolated from imported meat in Algeria. The genome sizes ranged from 4,601,209 to 4,958,962 bp. Antimicrobial resistance (AMR) genes, plasmids, and virulence factors were detected. ...[more]

PMID: 31467095

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Project description:Salmonella enterica is a foodborne zoonotic pathogen of significant public health concern. We have characterized the virulence and antimicrobial resistance gene content of 95 Salmonella isolates from 11 serovars by DNA microarray recovered from UK livestock or imported meat. Genes encoding resistance to sulphonamides (sul1, sul2), tetracycline [tet(A), tet(B)], streptomycin (strA, strB), aminoglycoside (aadA1, aadA2), beta-lactam (bla TEM), and trimethoprim (dfrA17) were common. Virulence gene content differed between serovars; S. Typhimurium formed two subclades based on virulence plasmid presence. Thirteen isolates were selected by their virulence profile for pathotyping using the Galleria mellonella pathogenesis model. Infection with a chicken invasive S. Enteritidis or S. Gallinarum isolate, a multidrug resistant S. Kentucky, or a S. Typhimurium DT104 isolate resulted in high mortality of the larvae; notably presence of the virulence plasmid in S. Typhimurium was not associated with increased larvae mortality. Histopathological examination showed that infection caused severe damage to the Galleria gut structure. Enumeration of intracellular bacteria in the larvae 24 h post-infection showed increases of up to 7 log above the initial inoculum and transmission electron microscopy (TEM) showed bacterial replication in the haemolymph. TEM also revealed the presence of vacuoles containing bacteria in the haemocytes, similar to Salmonella containing vacuoles observed in mammalian macrophages; although there was no evidence from our work of bacterial replication within vacuoles. This work shows that microarrays can be used for rapid virulence genotyping of S. enterica and that the Galleria animal model replicates some aspects of Salmonella infection in mammals. These procedures can be used to help inform on the pathogenicity of isolates that may be antibiotic resistant and have scope to aid the assessment of their potential public and animal health risk.

Dataset Information

Whole-Genome Sequencing of Six Strains of Salmonella enterica Isolated from Imported Meat in Algeria.

Publications

Whole-Genome Sequencing of Six Strains of Salmonella enterica Isolated from Imported Meat in Algeria.

Similar Datasets

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