Dataset Information

Identification of major consensus QTLs for seed size and minor QTLs for pod traits in cultivated groundnut (Arachis hypogaea L.).

ABSTRACT: Hundred kernel weight is an important indicator for large-seeded genotype selection. A recombinant inbred line population was used to decipher the genetic architecture of seed size and three pod traits in cultivated groundnut based on the phenotypic data from six and three environments, respectively. The study revealed a consensus major QTL for HKW in B07 group that explained 10.5-23.9% phenotypic variation due to seed size. Further, two other minor QTLs were identified in B03 and B08 group for the seed size. Two minor QTLs for pod beak were positioned in B03 and A08. A minor QTL for pod reticulation was also mapped in the same map interval with the pod beak QTL in A08. Another minor QTL for pod constriction was co-mapped with the minor QTL for HKW in B08. The other minor QTL for pod constriction was placed in the neighboring map interval with the consensus QTL for seed size in B07 that suggests linkage of pod constriction with large seed trait. Analysis of the flanking markers profile in 71 cultivated groundnut genotypes revealed a strong association of pPGPseq_2E06 marker with large seed trait.

SUBMITTER: Mondal S

PROVIDER: S-EPMC6717230 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Identification of major consensus QTLs for seed size and minor QTLs for pod traits in cultivated groundnut (<i>Arachis hypogaea</i> L.).

Mondal Suvendu S Badigannavar Anand M AM

3 Biotech 20190830 9

Hundred kernel weight is an important indicator for large-seeded genotype selection. A recombinant inbred line population was used to decipher the genetic architecture of seed size and three pod traits in cultivated groundnut based on the phenotypic data from six and three environments, respectively. The study revealed a consensus major QTL for HKW in B07 group that explained 10.5-23.9% phenotypic variation due to seed size. Further, two other minor QTLs were identified in B03 and B08 group for ...[more]

PMID: 31497465

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Project description:BackgroundCultivated peanut or groundnut (Arachis hypogaea L.) is the fourth most important oilseed crop in the world, grown mainly in tropical, subtropical and warm temperate climates. Due to its origin through a single and recent polyploidization event, followed by successive selection during breeding efforts, cultivated groundnut has a limited genetic background. In such species, microsatellite or simple sequence repeat (SSR) markers are very informative and useful for breeding applications. The low level of polymorphism in cultivated germplasm, however, warrants a need of larger number of polymorphic microsatellite markers for cultivated groundnut.ResultsA microsatellite-enriched library was constructed from the genotype TMV2. Sequencing of 720 putative SSR-positive clones from a total of 3,072 provided 490 SSRs. 71.2% of these SSRs were perfect type, 13.1% were imperfect and 15.7% were compound. Among these SSRs, the GT/CA repeat motifs were the most common (37.6%) followed by GA/CT repeat motifs (25.9%). The primer pairs could be designed for a total of 170 SSRs and were optimized initially on two genotypes. 104 (61.2%) primer pairs yielded scorable amplicon and 46 (44.2%) primers showed polymorphism among 32 cultivated groundnut genotypes. The polymorphic SSR markers detected 2 to 5 alleles with an average of 2.44 per locus. The polymorphic information content (PIC) value for these markers varied from 0.12 to 0.75 with an average of 0.46. Based on 112 alleles obtained by 46 markers, a phenogram was constructed to understand the relationships among the 32 genotypes. Majority of the genotypes representing subspecies hypogaea were grouped together in one cluster, while the genotypes belonging to subspecies fastigiata were grouped mainly under two clusters.ConclusionNewly developed set of 104 markers extends the repertoire of SSR markers for cultivated groundnut. These markers showed a good level of PIC value in cultivated germplasm and therefore would be very useful for germplasm analysis, linkage mapping, diversity studies and phylogenetic relationships in cultivated groundnut as well as related Arachis species.

Dataset Information

Identification of major consensus QTLs for seed size and minor QTLs for pod traits in cultivated groundnut (Arachis hypogaea L.).

Publications

Identification of major consensus QTLs for seed size and minor QTLs for pod traits in cultivated groundnut (<i>Arachis hypogaea</i> L.).

Similar Datasets

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