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Development of a multiplex fully automated assay for rapid quantification of CD4+ T cells from whole blood.

ABSTRACT: The development of cost-effective and rapid assays for the accurate counting of CD4 cells has remained prime focus for disease management. The lack of such assays has severely affected people living in resource-limited disease prevalent areas. CD4 count information plays a vital role in the effective management of HIV disease. There is an unmet need to develop rapid, cost-effective, portable and user-friendly point-of-care (POC) disease diagnostic platform technology for CD4+ T cell counting. Here, we have developed a flow-free magnetic actuation platform that uses antibody-coated magnetic beads to efficiently capture CD4+ T cells from a 30??L drop of whole blood. On-chip cell lysate electrical impedance spectroscopy has been utilized to quantify the isolated CD4 cells. The developed assay has a limit of detection of 25?cells per ?L and provides accurate CD4 counts in the range of 25-800?cells per ?L. The whole immunoassay along with the enumeration process is very rapid and provides CD4 quantification results within 5?min time frame. The assay does not require off-chip sample preparation steps and minimizes human involvement to a greater extent. The developed impedance-based immunoassay has potential to significantly improve the CD4 enumeration process especially for POC settings.

SUBMITTER: Sher M

PROVIDER: S-EPMC6718319 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Development of a multiplex fully automated assay for rapid quantification of CD4<sup>+</sup> T cells from whole blood.

Sher Mazhar M Asghar Waseem W

Biosensors & bioelectronics 20190702

The development of cost-effective and rapid assays for the accurate counting of CD4 cells has remained prime focus for disease management. The lack of such assays has severely affected people living in resource-limited disease prevalent areas. CD4 count information plays a vital role in the effective management of HIV disease. There is an unmet need to develop rapid, cost-effective, portable and user-friendly point-of-care (POC) disease diagnostic platform technology for CD4<sup>+</sup> T cell c ...[more]

PMID: 31302394

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Development of a multiplex fully automated assay for rapid quantification of CD4+ T cells from whole blood.

Publications

Development of a multiplex fully automated assay for rapid quantification of CD4<sup>+</sup> T cells from whole blood.

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