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Synthesis of water-soluble fluorescent polymeric glycoconjugate for the detection of cholera toxin.

ABSTRACT: Considering inherence optical properties of adjoint polyfluorenes and special functions of water-soluble conjugated glycopolymers, a triazole chain glycoconjugate via one-pot method were rapidly synthesized to prepare a lactate ligand polyfluorene with a clear fluorescent label by a nickel-catalyzed Yamamoto coupling polymerization. The water solubility and biocompatibility of the glycoconjugated polymer were ameliorated when the lactose group introduced as the side chain of the conjugated polymer. As a fluorescent multivalent system of glycoconjugates containing pyranogalactose groups, the interaction between pyranogalactose group and cholera toxin B subunit was studied by fluorescence spectrophotometric titration. PF-Lac has a broad application prospect in the check of cholera toxin and the study of glycoprotein interaction.

SUBMITTER: Feng L

PROVIDER: S-EPMC6719259 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Synthesis of water-soluble fluorescent polymeric glycoconjugate for the detection of cholera toxin.

Feng Lijuan L Zhong Mingjun M Zhang Shizhen S Wang Min M Sun Zhi-Yong ZY Chen Qi Q

Designed monomers and polymers 20190826 1

Considering inherence optical properties of adjoint polyfluorenes and special functions of water-soluble conjugated glycopolymers, a triazole chain glycoconjugate via one-pot method were rapidly synthesized to prepare a lactate ligand polyfluorene with a clear fluorescent label by a nickel-catalyzed Yamamoto coupling polymerization. The water solubility and biocompatibility of the glycoconjugated polymer were ameliorated when the lactose group introduced as the side chain of the conjugated polym ...[more]

PMID: 31496925

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