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The Preconditioning of Busulfan Promotes Efficiency of Human CD133+ Cells Engraftment in NOD Shi-SCID IL2R?cnull (NOG) Mice via Intra-Bone Marrow Injection.


ABSTRACT: Human CD133+ stem cells were injected into the bone marrow cavity of NOG (NOD Shi-SCID IL2R?cnull) mice with or without preconditioning of busulfan in order to assess the efficiency of human CD133+ cells engraftment. Peripheral blood from CD133+-engrafted NOG mice was analyzed by flow cytometry. The results showed that human CD19+ B lymphocytes could be detected at 4 weeks post-transplantation, and human CD4+, CD8+ subsets of T lymphocytes, CD19- CD14- HLA-DR+ DCs and CD19- CD14+ monocytes could be detected at 16 weeks post-transplantation. The survival rate of mice in busulfan-untreated group (100%) was slightly higher than that in the busulfan-pretreated group (83%) (P > 0.05). However, the differentiation efficiency of CD133+ stem cells in busulfan-pretreated group was significantly higher than that in the untreated group (P < 0.05). This data imply that CD133+ cells could be a good resource for a humanized mouse model, and the preconditioning of busulfan could be more conducive to accelerating the differentiation of human CD133+ cells in NOG mice by intra-bone marrow injection.

SUBMITTER: Guo X 

PROVIDER: S-EPMC6719503 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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The Preconditioning of Busulfan Promotes Efficiency of Human CD133+ Cells Engraftment in NOD Shi-SCID IL2Rγcnull (NOG) Mice via Intra-Bone Marrow Injection.

Guo Xiaofang X   Yin Xiaoxiao X   Zhu Wenjuan W   Pan Ying Y   Wang Hui H   Liang Yinming Y   Zhu Xiaofei X  

Cell transplantation 20190415 7


Human CD133<sup>+</sup> stem cells were injected into the bone marrow cavity of NOG (NOD Shi-SCID IL2Rγc<sup>null</sup>) mice with or without preconditioning of busulfan in order to assess the efficiency of human CD133<sup>+</sup> cells engraftment. Peripheral blood from CD133<sup>+</sup>-engrafted NOG mice was analyzed by flow cytometry. The results showed that human CD19<sup>+</sup> B lymphocytes could be detected at 4 weeks post-transplantation, and human CD4<sup>+</sup>, CD8<sup>+</sup> subs  ...[more]

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