Hybrids made from antimicrobial peptides with different mechanisms of action show enhanced membrane permeabilization.

ABSTRACT: Combining two known antimicrobial peptides (AMPs) into a hybrid peptide is one promising avenue in the design of agents with increased antibacterial activity. However, very few previous studies have considered the effect of creating a hybrid from one AMP that permeabilizes membranes and another AMP that acts intracellularly after translocating across the membrane. Moreover, very few studies have systematically evaluated the order of parent peptides or the presence of linkers in the design of hybrid AMPs. Here, we use a combination of antibacterial measurements, cellular assays and semi-quantitative confocal microscopy to characterize the activity and mechanism for a library of sixteen hybrid peptides. These hybrids consist of permutations of two primarily membrane translocating peptides, b

SUBMITTER: Wade HM 

PROVIDER: S-EPMC6721965 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature