Dataset Information

Assessing Anthocyanin Biosynthesis in Solanaceae as a Model Pathway for Secondary Metabolism.

ABSTRACT: Solanaceae have played an important role in elucidating how flower color is specified by the flavonoid biosynthesis pathway (FBP), which produces anthocyanins and other secondary metabolites. With well-established reverse genetics tools and rich genomic resources, Solanaceae provide a robust framework to examine the diversification of this well-studied pathway over short evolutionary timescales and to evaluate the predictability of genetic perturbation on pathway flux. Genomes of eight Solanaceae species, nine related asterids, and four rosids were mined to evaluate variation in copy number of the suite of FBP enzymes involved in anthocyanin biosynthesis. Comparison of annotation sources indicated that the NCBI annotation pipeline generated more and longer FBP annotations on average than genome-specific annotation pipelines. The pattern of diversification of each enzyme among asterids was assessed by phylogenetic analysis, showing that the CHS superfamily encompasses a large paralogous family of ancient and recent duplicates, whereas other FBP enzymes have diversified via recent duplications in particular lineages. Heterologous expression of a pansy F3'5'H gene in tobacco changed flower color from pink to dark purple, demonstrating that anthocyanin production can be predictably modified using reverse genetics. These results suggest that the Solanaceae FBP could be an ideal system to model genotype-to-phenotype interactions for secondary metabolism.

SUBMITTER: Li Z

PROVIDER: S-EPMC6723469 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Assessing Anthocyanin Biosynthesis in Solanaceae as a Model Pathway for Secondary Metabolism.

Li Zuo Z Vickrey Trisha L TL McNally Moira G MG Sato Shirley J SJ Clemente Tom Elmo TE Mower Jeffrey P JP

Genes 20190725 8

Solanaceae have played an important role in elucidating how flower color is specified by the flavonoid biosynthesis pathway (FBP), which produces anthocyanins and other secondary metabolites. With well-established reverse genetics tools and rich genomic resources, Solanaceae provide a robust framework to examine the diversification of this well-studied pathway over short evolutionary timescales and to evaluate the predictability of genetic perturbation on pathway flux. Genomes of eight Solanacea ...[more]

PMID: 31349565

Similar Datasets

Project description:Deficit irrigation (DI) is an irrigation scheduling technique that is used in grapes to improve red color development; however, results are not always satisfactory in table grapes. The red color in grapes is mainly due to the plant pigment anthocyanin. In the present study, the anthocyanin biosynthesis in Scarlet Royal grapes (Vitis vinifera L.) grown in the San Joaquin and Coachella Valleys, and subjected to two different DI strategies was investigated. The objective of this study was to identify potential regulatory factors that may lead to potential treatments to improve red color in table grapes, especially under warm climate conditions. In both locations, DI induced the expression of several genes involved in three major pathways that control the red color in table grapes: anthocyanin biosynthesis, hormone biosynthesis, and antioxidant system. DI at veraison induced anthocyanin accumulation and enhanced red color in berries at harvest time. However, anthocyanin accumulation was lower at the Coachella Valley compared to the San Joaquin Valley. The lower level of anthocyanin was associated with lower expression of critical genes involved in anthocyanin biosynthesis, such as flavonoid-3-O-glucosyltransferase (UFGT), myb-related regulatory gene (R2R3-MYB) (MYBA1), basic helix-loop-helix (bHLH) (MYCA1) and the tryptophan-aspartic acid repeat (WDR or WD40) proteins (WDR1). Further, gene expression analysis revealed the association of ABA biosynthesis gene 9-cis-epoxycarotenoid dioxygenase (NCED1), 1-aminocyclopropane-1-carboxylic acid oxidase (ACO3), and the gibberellic acid (GA) catabolic gene GA2 oxidase (GA2ox1) in the induction of anthocyanin biosynthesis. An increase in the chalcone synthase gene (CHS2) was observed in response to DI treatments in both sites. However, CHS2 expression was higher in Coachella Valley after ending the DI treatment, suggesting the involvement of environmental stress in elevating its transcripts. This data was also supported by the lower level of antioxidant gene expression and enzyme activities in the Coachella Valley compared to the San Joaquin Valley. The present data suggested that the lack of grape red coloration could partially be due to the lower level of antioxidant activities resulting in accelerated anthocyanin degradation and impaired anthocyanin biosynthesis. It seems that under challenging warmer conditions, several factors are required to optimize anthocyanin accumulation via DI, including an active antioxidant system, proper light perception, and hormonal balance.

Dataset Information

Assessing Anthocyanin Biosynthesis in Solanaceae as a Model Pathway for Secondary Metabolism.

Publications

Assessing Anthocyanin Biosynthesis in Solanaceae as a Model Pathway for Secondary Metabolism.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets