Quality Evaluation of Oviductus Ranae Based on PUFAs Using HPLC Fingerprint Techniques Combined with Chemometric Methods.

ABSTRACT: Oviductus Ranae is a nutritional product for both medicine and food. Its quality is uneven due to multiple factors. An efficient method was established to evaluate the quality of Oviductus Ranae using fingerprint techniques and chemometric methods based on the analysis of polyunsaturated fatty acids (PUFAs) in petroleum ether extract by high performance liquid chromatography (HPLC). During this process, 27 batches of Oviductus Ranae were analyzed by HPLC and two types of chromatographic fingerprints were established. The fingerprint that was obtained by matching six known peaks was used for the quantification of six PUFAs. Another fingerprint was obtained by matching sixteen peaks with a peak area ratio greater than 0.5% and it was used to classify the different qualities of Oviductus Rana

SUBMITTER: Guo H 

PROVIDER: S-EPMC6723783 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature