Ontology highlight
ABSTRACT:
SUBMITTER: Baldering TN
PROVIDER: S-EPMC6724688 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Baldering Tim N TN Dietz Marina S MS Gatterdam Karl K Karathanasis Christos C Wieneke Ralph R Tampé Robert R Heilemann Mike M
Molecular biology of the cell 20190410 12
How membrane proteins oligomerize determines their function. Superresolution microscopy can report on protein clustering and extract quantitative molecular information. Here, we evaluate the blinking kinetics of four photoactivatable fluorescent proteins for quantitative single-molecule microscopy. We identified mEos3.2 and mMaple3 to be suitable for molecular quantification through blinking histogram analysis. We designed synthetic and genetic dimers of mEos3.2 as well as fusion proteins of mon ...[more]