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A functional role for small-conductance calcium-activated potassium channels in sensory pathways including nociceptive processes.


ABSTRACT: We investigated the role of small-conductance calcium-activated potassium (SK) and intermediate-conductance calcium-activated potassium channels in modulating sensory transmission from peripheral afferents into the rat spinal cord. Subunit-specific antibodies reveal high levels of SK3 immunoreactivity in laminas I, II, and III of the spinal cord. Among dorsal root ganglion neurons, both peripherin-positive (C-type) and peripherin-negative (A-type) cells show intense SK3 immunoreactivity. Furthermore, dorsal root-stimulated sensory responses recorded in vitro are inhibited when SK channel activity is increased with 1-ethyl-2-benzimidazolinone (1-EBIO). In vivo electrophysiological recordings show that neuronal responses to naturally evoked nociceptive and nonnociceptive stimuli increase after application of the selective SK channel blocker 8,14-diaza-1,7(1,4)-diquinolinacyclotetradecaphanedium di-trifluoroacetate (UCL 1848), indicating that SK channels are normally active in moderating afferent input. Conversely, neuronal responses evoked by mechanical stimuli are inhibited when SK channel activity is increased with 1-EBIO. These effects are reversed by the subsequent application of UCL 1848. Our data demonstrate that SK channels have an important role in controlling sensory input into the spinal cord.

SUBMITTER: Bahia PK 

PROVIDER: S-EPMC6725366 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature

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A functional role for small-conductance calcium-activated potassium channels in sensory pathways including nociceptive processes.

Bahia Parmvir K PK   Suzuki Rie R   Benton David C H DC   Jowett Amanda J AJ   Chen Mao Xiang MX   Trezise Derek J DJ   Dickenson Anthony H AH   Moss Guy W J GW  

The Journal of neuroscience : the official journal of the Society for Neuroscience 20050401 14


We investigated the role of small-conductance calcium-activated potassium (SK) and intermediate-conductance calcium-activated potassium channels in modulating sensory transmission from peripheral afferents into the rat spinal cord. Subunit-specific antibodies reveal high levels of SK3 immunoreactivity in laminas I, II, and III of the spinal cord. Among dorsal root ganglion neurons, both peripherin-positive (C-type) and peripherin-negative (A-type) cells show intense SK3 immunoreactivity. Further  ...[more]

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