Ontology highlight
ABSTRACT:
SUBMITTER: Benaoudia S
PROVIDER: S-EPMC6727027 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Benaoudia Sacha S Martin Amandine A Puig Gamez Marta M Gay Gabrielle G Lagrange Brice B Cornut Maxence M Krasnykov Kyrylo K Claude Jean-Baptiste JB Bourgeois Cyril F CF Hughes Sandrine S Gillet Benjamin B Allatif Omran O Corbin Antoine A Ricci Romeo R Henry Thomas T
EMBO reports 20190729 9
Caspase-4, the cytosolic LPS sensor, and gasdermin D, its downstream effector, constitute the non-canonical inflammasome, which drives inflammatory responses during Gram-negative bacterial infections. It remains unclear whether other proteins regulate cytosolic LPS sensing, particularly in human cells. Here, we conduct a genome-wide CRISPR/Cas9 screen in a human monocyte cell line to identify genes controlling cytosolic LPS-mediated pyroptosis. We find that the transcription factor, IRF2, is req ...[more]