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Analysis of widely targeted metabolites of the euhalophyte Suaeda salsa under saline conditions provides new insights into salt tolerance and nutritional value in halophytic species.

ABSTRACT: BACKGROUND:Suaeda salsa L. (S. salsa) is an annual euhalophyte with high salt tolerance and high value as an oil crop, traditional Chinese medicine and vegetable. However, there are few comprehensive studies on the metabolomics of S. salsa under saline conditions. RESULTS:Seedlings of S. salsa were cultured with 0, 200 and 500 mM NaCl for two days. Then, widely targeted metabolites were detected with ultra performance liquid chromatography and tandem mass spectrometry. A total of 639 metabolites were annotated. Among these, 253 metabolites were differential metabolites. Salt treatment increased the content of certain metabolites, such as nucleotide and its derivates, organic acids, the content of amino acids, lipids such as α-linolenic acid, and certain antioxidants such as quercetin. These substances may be correlated to osmotic tolerance, increased antioxidant activity, and medical and nutritional value in the species. CONCLUSION:This study comprehensively analyzed the metabolic response of S. salsa under salinity from the perspective of omics, and provides an important theoretical basis for understanding salt tolerance and evaluating nutritional value in the species.

SUBMITTER: Li Q

PROVIDER: S-EPMC6729093 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Cation and Zn Accumulation in Brown Seeds of the Euhalophyte <i>Suaeda salsa</i> Improves Germination Under Saline Conditions.

Project description:Salinity inhibits plant growth due to salt ion accumulation in plant cells and reduced absorption of other nutrients such as metal ions; however halophyte plants have evolved mechanisms to survive and thrive in high-salt conditions. The euhalophyte Suaeda salsa generates dimorphic seeds (black and brown), which show marked differences in germination and seedling growth under high-salt conditions. However, it is unclear whether their ionic status differs. Here, to provide insight on the role of ions in salt tolerance, we used inductively coupled plasma mass spectrometry to measure the ion contents in the dimorphic seeds from S. salsa plants treated with or without NaCl. We measured the macroelements Na, K, Mg, and Ca, and the microelements Mn, Fe, Zn, Cu, and Mo. NaCl-treated S. salsa plants produced seeds with significantly reduced metallic element contents and significantly increased Na+ contents. The brown seeds of S. salsa plants treated with 0 and 200 mM NaCl had much higher contents of K+, Ca2+, and Fe2+ compared with the black seeds. However, the S. salsa seeds (both black and brown) from NaCl-treated plants were significantly larger, and had higher germination rate and higher seedling salt tolerance compared with seeds from plants not treated with NaCl. Interestingly, we measured significantly higher Zn2+ contents in the brown seeds from plants treated with NaCl compared with the black seeds. This suggests that the high contents of Zn2+ and other cations affected seed development and salt tolerance during germination under high-salt conditions. These observations provide insight into the mechanisms of salt tolerance in this halophyte and inform efforts to increase salt tolerance in salt-sensitive species.

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Transcriptomic profiling of genes in matured dimorphic seeds of euhalophyte Suaeda salsa.

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NaCl improves reproduction by enhancing starch accumulation in the ovules of the euhalophyte Suaeda salsa.

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Overexpression of Glycerol-3-Phosphate Acyltransferase from Suaeda salsa Improves Salt Tolerance in Arabidopsis.

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