Amyloid-like substance in mice and human oocytes and embryos.

ABSTRACT:

Purpose

To identify and characterize amyloid-like substance (ALS) in human and mouse oocytes and preimplantation embryos.

Methods

An experimental prospective pilot study. A total of 252 mouse oocytes and preimplantation embryos and 50 immature and in vitro matured human oocytes and parthenogenetic human embryos, from 11 consenting fertility patients, ages 18-45. Fluorescence intensity from immunofluorescent staining and data from confocal microscopy were quantified. Data were compared by one-way analysis of variance, with the least square-MEANS post-test, Pearson correlation coefficients (r), and bivariate analyses (t tests). ALS morphology was verified using transmission electron microscopy.

Results

Immunostaining for ALS appears throughout the zona pellucida, as wel

SUBMITTER: Pimentel RN 

PROVIDER: S-EPMC6730981 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature