Identification and characterization of a hyperthermophilic GH9 cellulase from the Arctic Mid-Ocean Ridge vent field.
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ABSTRACT:
SUBMITTER: Stepnov AA
PROVIDER: S-EPMC6731012 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Publications
Identification and characterization of a hyperthermophilic GH9 cellulase from the Arctic Mid-Ocean Ridge vent field.
Stepnov Anton A AA Fredriksen Lasse L Steen Ida H IH Stokke Runar R Eijsink Vincent G H VGH
PloS one 20190906 9
A novel GH9 cellulase (AMOR_GH9A) was discovered by sequence-based mining of a unique metagenomic dataset collected at the Jan Mayen hydrothermal vent field. AMOR_GH9A comprises a signal peptide, a catalytic domain and a CBM3 cellulose-binding module. AMOR_GH9A is an exceptionally stable enzyme with a temperature optimum around 100°C and an apparent melting temperature of 105°C. The novel cellulase retains 64% of its activity after 4 hours of incubation at 95°C. The closest characterized homolog ...[more]
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