Ontology highlight
ABSTRACT:
SUBMITTER: Low JH
PROVIDER: S-EPMC6731150 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Low Jian Hui JH Li Pin P Chew Elaine Guo Yan EGY Zhou Bingrui B Suzuki Keiichiro K Zhang Tian T Lian Michelle Mulan MM Liu Meng M Aizawa Emi E Rodriguez Esteban Concepcion C Yong Kylie Su Mei KSM Chen Qingfeng Q Campistol Josep M JM Fang Mingliang M Khor Chiea Chuen CC Foo Jia Nee JN Izpisua Belmonte Juan Carlos JC Xia Yun Y
Cell stem cell 20190711 3
Human pluripotent stem cell-derived kidney organoids recapitulate developmental processes and tissue architecture, but intrinsic limitations, such as lack of vasculature and functionality, have greatly hampered their application. Here we establish a versatile protocol for generating vascularized three-dimensional (3D) kidney organoids. We employ dynamic modulation of WNT signaling to control the relative proportion of proximal versus distal nephron segments, producing a correlative level of vasc ...[more]