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Hepatitis C virus (HCV) genotype 1b displays higher genetic variability of hypervariable region 1 (HVR1) than genotype 3.

ABSTRACT: Hepatitis C virus (HCV) is characterized by high genetic variability, which is manifested both at the inter-host and intra-host levels. However, its role in the clinical course of infection is less obvious. The aim of the present study was to determine the genetic variability of HCV HVR1 (hypervariable region 1) of genotype 1b and 3 in plasma of blood donors in the early seronegative stage of infection (HCV-RNA+, anti-HCV-) and in samples from chronically infected patients using next-generation sequencing. Sequencing errors were corrected, and haplotypes inferred using the ShoRAH software. Genetic diversity parameters (intra-host number of variants, number of nucleotide substitutions and diversity per site) were assessed by DNA SP and MEGA. During the early infection, the number of variants were significantly lower in subjects infected with genotype 3 than with genotype 1b (p?

SUBMITTER: Janiak M 

PROVIDER: S-EPMC6731259 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Hepatitis C virus (HCV) genotype 1b displays higher genetic variability of hypervariable region 1 (HVR1) than genotype 3.

Janiak Maciej M   Perlejewski Karol K   Grabarczyk Piotr P   Kubicka-Russel Dorota D   Zagordi Osvaldo O   Berak Hanna H   Osuch Sylwia S   Pawełczyk Agnieszka A   Bukowska-Ośko Iwona I   Płoski Rafał R   Laskus Tomasz T   Caraballo Cortés Kamila K  

Scientific reports 20190906 1

Hepatitis C virus (HCV) is characterized by high genetic variability, which is manifested both at the inter-host and intra-host levels. However, its role in the clinical course of infection is less obvious. The aim of the present study was to determine the genetic variability of HCV HVR1 (hypervariable region 1) of genotype 1b and 3 in plasma of blood donors in the early seronegative stage of infection (HCV-RNA+, anti-HCV-) and in samples from chronically infected patients using next-generation  ...[more]