Investigation of the Amide Proton Solvent Exchange Properties of Glycosaminoglycan Oligosaccharides.

ABSTRACT: One-dimensional ¹H NMR experiments were conducted for aqueous solutions of glycosaminoglycan oligosaccharides to measure the amide proton temperature coefficients and activation energy barriers for solvent exchange and evaluate the effect of pH on the solvent exchange properties. A library of mono- and oligosaccharides was prepared by enzymatic depolymerization of amide-containing polysaccharides and by chemical modification of heparin and heparan sulfate saccharides including members that contain a 3- O-sulfated glucosamine residue. The systematic evaluation of this saccharide library facilitated assessment of the effects of structural characteristics, such as size, sulfation number and site, and glycosidic linkage, on amide proton solvent exchange rates. Charge repulsion by ne

SUBMITTER: Green AR 

PROVIDER: S-EPMC6732216 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature