Dataset Information

Dendrobium alkaloids decrease A? by regulating ?- and ?-secretases in hippocampal neurons of SD rats.

ABSTRACT:

Background

Alzheimer's disease (AD) is the primary cause of dementia in the elderly. The imbalance between production and clearance of amyloid ? (A?) is a very early, often initiating factor in AD. Dendrobium nobile Lindl. alkaloids (DNLA) extracted from a Chinese medicinal herb, which have been shown to have anti-aging effects, protected against neuronal impairment in vivo and in vitro. Moreover, we confirmed that DNLA can improve learning and memory function in elderly normal mice, indicating that DNLA has potential health benefits. However, the underlying mechanism is unclear. Therefore, we further explored the effect of DNLA on neurons, which is closely related to learning and memory, based on A?.

Methods

We exposed cultured hippocampal neurons to DNLA to investigate the effect of DNLA on A? in vitro. Cell viability was evaluated by MTT assays. Proteins were analyzed by Western blot analysis.

Results

The cell viability of hippocampal neurons was not changed significantly after treatment with DNLA. But DNLA reduced the protein expression of amyloid precursor protein (APP), disintegrin and metalloprotease 10 (ADAM10), ?-site APP cleaving enzyme 1 (BACE1) and A?_1-42 of hippocampal neurons in rats and increased the protein expression of ADAM17.

Conclusions

DNLA decreases A? by regulating ?- and ?-secretase in hippocampal neurons of SD rats.

SUBMITTER: Huang J

PROVIDER: S-EPMC6733236 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

<i>Dendrobium</i> alkaloids decrease Aβ by regulating α- and β-secretases in hippocampal neurons of SD rats.

Huang Juan J Huang Nanqu N Zhang Minghui M Nie Jing J Xu Yunyan Y Wu Qin Q Shi Jingshan J

PeerJ 20190906

<h4>Background</h4>Alzheimer's disease (AD) is the primary cause of dementia in the elderly. The imbalance between production and clearance of amyloid β (Aβ) is a very early, often initiating factor in AD. <i>Dendrobium nobile</i> Lindl. alkaloids (DNLA) extracted from a Chinese medicinal herb, which have been shown to have anti-aging effects, protected against neuronal impairment <i>in vivo</i> and <i>in vitro</i>. Moreover, we confirmed that DNLA can improve learning and memory function in eld ...[more]

PMID: 31534855

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