An Improved Method of Maintaining Primary Murine Cardiac Fibroblasts in Two-Dimensional Cell Culture.
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ABSTRACT:
SUBMITTER: Landry NM
PROVIDER: S-EPMC6733858 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Publications
An Improved Method of Maintaining Primary Murine Cardiac Fibroblasts in Two-Dimensional Cell Culture.
Landry Natalie M NM Rattan Sunil G SG Dixon Ian M C IMC
Scientific reports 20190909 1
Primary cardiac fibroblasts are notoriously difficult to maintain for extended periods of time in cell culture, due to the plasticity of their phenotype and sensitivity to mechanical input. In order to study cardiac fibroblast activation in vitro, we have developed cell culture conditions which promote the quiescent fibroblast phenotype in primary cells. Using elastic silicone substrata, both rat and mouse primary cardiac fibroblasts could be maintained in a quiescent state for more than 3 days ...[more]
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