Characterizing an engineered carotenoid-producing yeast as an anti-stress chassis for building cell factories.

ABSTRACT:

Background

A microorganism engineered for non-native tasks may suffer stresses it never met before. Therefore, we examined whether a Kluyveromyces marxianus strain engineered with a carotenoid biosynthesis pathway can serve as an anti-stress chassis for building cell factories.

Results

Carotenoids, a family of antioxidants, are valuable natural products with high commercial potential. We showed that the free radical removal ability of carotenoids can confer the engineered host with a higher tolerance to ethanol, so that it can produce more bio-ethanol than the wild type. Moreover, we found that this engineered strain has improved tolerance to other toxic effects including furfurals, heavy metals such as arsenate (biomass contaminant) and isobutanol (end product). Furthermore

SUBMITTER: Liu HL 

PROVIDER: S-EPMC6737703 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature