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Exposure to a 50 Hz magnetic field at 100 µT exerts no DNA damage in cardiomyocytes.

ABSTRACT: The effects of exposure to magnetic fields (MFs) at electric frequencies (50-60 Hz) on carcinogenicity are still in debate. Whether exposure to MFs affects the heart is also a debated issue. This study aimed to determine whether exposure to extremely low frequency MFs (ELF-MFs) induced DNA damage in cardiomyocytes both in vitro and in vivo Human ventricular cardiomyocytes were exposed to 50 Hz ELF-MF at 100 µT for 1 h continuously or 75 min intermittently. The effects of the treatments were evaluated by DNA damage, redox status changes and relative signal molecular expression. Moreover, ten male Sprague-Dawley rats were exposed to a 50 Hz MF at 100 µT for 7 days, while another 10 rats were sham exposed. The protein levels of p53 and Hsp70 in heart tissue were analyzed by western blot. The results showed that exposure to ELF-MF did not induce DNA damage, changes to cell cycle distribution or increased reactive oxygen species level. No significant differences were detected in p53 and Hsp70 expression level between the ELF-MF and sham-exposure groups both in vitro and in vivo All these data indicate that MFs at power-frequency may not cause DNA damage in cardiomyocytes.This article has an associated First Person interview with the first author of the paper.

SUBMITTER: Wang Y

PROVIDER: S-EPMC6737969 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Exposure to a 50 Hz magnetic field at 100 µT exerts no DNA damage in cardiomyocytes.

Wang Yong Y Liu Xingfa X Zhang Yemao Y Wan Baoquan B Zhang Jiangong J He Wei W Hu Dong D Yang Yong Y Lai Jinsheng J He Mengying M Chen Chen C

Biology open 20190815 8

The effects of exposure to magnetic fields (MFs) at electric frequencies (50-60 Hz) on carcinogenicity are still in debate. Whether exposure to MFs affects the heart is also a debated issue. This study aimed to determine whether exposure to extremely low frequency MFs (ELF-MFs) induced DNA damage in cardiomyocytes both <i>in vitro</i> and <i>in vivo</i> Human ventricular cardiomyocytes were exposed to 50 Hz ELF-MF at 100 µT for 1 h continuously or 75 min intermittently. The effects of the treatm ...[more]

PMID: 31362949

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Project description:Consistent and independently replicated laboratory evidence to support a causative relationship between environmental exposure to extremely low frequency electromagnetic fields (ELF-EMF) at power line frequencies and the associated increase in risk of childhood leukaemia has not been obtained. In particular, although gene expression responses have been reported in a wide variety of cells, none have emerged as robust, widely replicated effects. DNA microarrays facilitate comprehensive searches for changes in gene expression without a requirement to select candidate responsive genes. To determine if gene expression changes occur in white blood cells of volunteers exposed to an ELF-EMF, each of 17 pairs of male volunteers age 20-30 was subjected either to a 50 Hz EMF exposure of 62.0 ± 7.1 μT for 2 h or to a sham exposure (0.21 ± 0.05 μT) at the same time (11:00-13:00). The alternative regime for each volunteer was repeated on the following day and the two-day sequence was repeated 6 days later except that a null exposure (0.085 ± 0.01 μT) replaced the sham exposure. Five blood samples (10 ml) were collected at 2 h intervals from 9:00 to 17:00 with five additional samples during the exposure and sham or null exposure periods on each study day at 11.05, 11.10, 11.20, 11.40 and 12.20. RNA samples were pooled for the same time points on each study day for the 17 volunteers that were subjected to the ELF-EMF exposure/sham or null exposure sequence and were analysed on Illumina microarrays. Time courses for 16 mammalian genes previously reported to be responsive to ELF-EMF exposure, including immediate early genes, stress response, cell proliferation and apoptotic genes were examined in detail. No genes or gene sets significantly expressed on the arrays showed consistent response profiles to repeated ELF-EMF exposures. A stress response was detected as a transient increase in plasma cortisol at the onset of either exposure or sham exposure on the first study day. The cortisol response diminished progressively on subsequent exposures or sham exposures and was attributable to mild stress associated with the experimental protocol.

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Exposure to a 50 Hz magnetic field at 100 µT exerts no DNA damage in cardiomyocytes.

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Exposure to a 50 Hz magnetic field at 100 µT exerts no DNA damage in cardiomyocytes.

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