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An inexpensive microscopy system for microfluidic studies in budding yeast.

ABSTRACT: Recently, microfluidic technologies have been developed to allow higher throughput collection of yeast replicative lifespan data. Adoption of these devices has been limited, in part, due to the high cost of the motorized microscopy instrumentation from mainline manufacturers. Inspired by recent development of open source microscopy hardware and software, we developed minimal-cost hardware attachments to provide long-term focus stabilization for lower-cost microscopes and open source software to manage concurrent time-lapse image acquisition from multiple microscopes. We hope that these tools will help spur the wider adoption of microfluidic technologies for the study of aging in yeast.

SUBMITTER: Chen KL

PROVIDER: S-EPMC6738973 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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An inexpensive microscopy system for microfluidic studies in budding yeast.

Chen Kenneth L KL Ven Toby N TN Crane Matthew M MM Chen Dexter E DE Feng Yen-Chi YC Suzuki Nozomi N Russell Adam E AE de Moraes Diogo D Kaeberlein Matt M

Translational medicine of aging 20190607

Recently, microfluidic technologies have been developed to allow higher throughput collection of yeast replicative lifespan data. Adoption of these devices has been limited, in part, due to the high cost of the motorized microscopy instrumentation from mainline manufacturers. Inspired by recent development of open source microscopy hardware and software, we developed minimal-cost hardware attachments to provide long-term focus stabilization for lower-cost microscopes and open source software to ...[more]

PMID: 31511839

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