Dataset Information

Cartilage Damage Is Related to ACL Stiffness in a Porcine Model of ACL Repair.

ABSTRACT: Inferior anterior cruciate ligament (ACL) structural properties may inadequately restrain tibiofemoral joint motion following surgery, contributing to the increased risk of post-traumatic osteoarthritis. Using both a direct measure of ACL linear stiffness and an in vivo magnetic resonance imaging (MRI) T2 *-based prediction model, we hypothesized that cartilage damage and ACL stiffness would increase over time, and that an inverse relationship between cartilage damage and ACL stiffness would emerge at a later stage of healing. After either 6, 12, or 24 weeks (w) of healing after ACL repair, ACL linear stiffness was determined from the force-displacement relationship during tensile testing ex vivo and predicted in vivo from the MRI T2 *-based multiple linear regression model in 24 Yucatan minipigs. Tibiofemoral cartilage was graded postmortem. There was no relationship between cartilage damage and ACL stiffness at 6?w (R2 ?=?0.04; p?=?0.65), 12?w (R2 ?=?0.02; p?=?0.77), or when the data from all animals were pooled (R2 ?=?0.02; p?=?0.47). A significant inverse relationship between cartilage damage and ACL stiffness based on both ex vivo measurement (R2 ?=?0.90; p?

SUBMITTER: Beveridge JE

PROVIDER: S-EPMC6739195 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Cartilage Damage Is Related to ACL Stiffness in a Porcine Model of ACL Repair.

Beveridge Jillian E JE Proffen Benedikt L BL Karamchedu Naga Padmini NP Chin Kaitlyn E KE Sieker Jakob T JT Badger Gary J GJ Kiapour Ata M AM Murray Martha M MM Fleming Braden C BC

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 20190625 10

Inferior anterior cruciate ligament (ACL) structural properties may inadequately restrain tibiofemoral joint motion following surgery, contributing to the increased risk of post-traumatic osteoarthritis. Using both a direct measure of ACL linear stiffness and an in vivo magnetic resonance imaging (MRI) T<sub>2</sub> *-based prediction model, we hypothesized that cartilage damage and ACL stiffness would increase over time, and that an inverse relationship between cartilage damage and ACL stiffnes ...[more]

PMID: 31125133

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Project description:To analyze the genetic and biomolecular mechanisms underlying cartilage repair, an optimized mouse model of osteochondral repair is required. Although several models of articular cartilage injury in mice have recently been established, the articular surface in adult C57Bl/6 mice heals poorly. Since C57Bl/6 mice are the most popular strain of genetically manipulated mice, an articular cartilage repair model using C57Bl/6 mice would be helpful for analysis of the mechanisms of cartilage repair. The purpose of this study was to establish a cartilage repair model in C57Bl/6 mice using immature animals. To achieve this goal, full-thickness injuries were generated in 3-week-old (young), 4-week-old (juvenile), and 8-week-old (adult) C57Bl/6 mice. To investigate the reproducibility and consistency of full-thickness injuries, mice were sacrificed immediately after operation, and cartilage thickness at the patellar groove, depth of the cartilage injury, cross-sectional width, and cross-sectional area were compared among the three age groups. The depth of cartilage injury/cartilage thickness ratio (%depth) and the coefficient of variation (CV) for each parameter were also calculated. At 8 weeks postoperatively, articular cartilage repair was assessed using a histological scoring system. With respect to the reproducibility and consistency of full-thickness injuries, cartilage thickness, depth of cartilage injury, and cross-sectional area were significantly larger in young and juvenile mice than in adult mice, whereas cross-sectional width and %depth were almost equal among the three age groups. CVs of %depths were less than 10% in all groups. With respect to articular cartilage repair, young and juvenile mice showed superior results. In conclusion, we established a novel cartilage repair model in C57Bl/6 mice. This model will be valuable in achieving mechanistic insights into the healing process of the joint surface, as it will facilitate the use of genetically modified mice, which are most commonly developed on a C57Bl/6 background.

Dataset Information

Cartilage Damage Is Related to ACL Stiffness in a Porcine Model of ACL Repair.

Publications

Cartilage Damage Is Related to ACL Stiffness in a Porcine Model of ACL Repair.

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