Ontology highlight
ABSTRACT:
SUBMITTER: Partlow EA
PROVIDER: S-EPMC6739873 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Partlow Edward A EA Baker Richard W RW Beacham Gwendolyn M GM Chappie Joshua S JS Leschziner Andres E AE Hollopeter Gunther G
eLife 20190829
Endocytosis of transmembrane proteins is orchestrated by the AP2 clathrin adaptor complex. AP2 dwells in a closed, inactive state in the cytosol, but adopts an open, active conformation on the plasma membrane. Membrane-activated complexes are also phosphorylated, but the significance of this mark is debated. We recently proposed that NECAP negatively regulates AP2 by binding open and phosphorylated complexes (Beacham et al., 2018). Here, we report high-resolution cryo-EM structures of NECAP boun ...[more]