Castration Induces Down-Regulation of A-Type K⁺ Channel in Rat Vas Deferens Smooth Muscle.

ABSTRACT: A-type K⁺ channels contribute to regulating the propagation and frequency of action potentials in smooth muscle cells (SMCs). The present study (i) identified the molecular components of A-type K⁺ channels in rat vas deferens SMs (VDSMs) and (ii) showed the long-term, genomic effects of testosterone on their expression in VDSMs. Transcripts of the A-type K⁺ channel α subunit, Kv4.3L and its regulatory β subunits, KChIP3, NCS1, and DPP6-S were predominantly expressed in rat VDSMs over the other related subtypes (Kv4.2, KChIP1, KChIP2, KChIP4, and DPP10). A-type K⁺ current (I_A) density in VDSM cells (VDSMCs) was decreased by castration without changes in I_A kinetics, and decreased I_A density was compensated for by an oral treatment with 17α-methyltestosterone (MET). Correspondingly, in the VDSMs of castrated rats, Kv4.3L and KChIP3 were down-regulated at both the transcript and protein expression levels. Changes in Kv4.3L and KChIP3 expression levels were compensated for by the treatment with MET. These results suggest that testosterone level changes in testosterone disorders and growth processes control the functional expression of A-type K⁺ channels in VDSMCs.

SUBMITTER: Ohya S 

PROVIDER: S-EPMC6747096 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature