Multispectral tracing in densely labeled mouse brain with nTracer.
Ontology highlight
ABSTRACT:
SUBMITTER: Roossien DH
PROVIDER: S-EPMC6748755 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Publications
Multispectral tracing in densely labeled mouse brain with nTracer.
Roossien Douglas H DH Sadis Benjamin V BV Yan Yan Y Webb John M JM Min Lia Y LY Dizaji Aslan S AS Bogart Luke J LJ Mazuski Cristina C Huth Robert S RS Stecher Johanna S JS Akula Sriakhila S Shen Fred F Li Ye Y Xiao Tingxin T Vandenbrink Madeleine M Lichtman Jeff W JW Hensch Takao K TK Herzog Erik D ED Cai Dawen D
Bioinformatics (Oxford, England) 20190901 18
<h4>Summary</h4>This note describes nTracer, an ImageJ plug-in for user-guided, semi-automated tracing of multispectral fluorescent tissue samples. This approach allows for rapid and accurate reconstruction of whole cell morphology of large neuronal populations in densely labeled brains.<h4>Availability and implementation</h4>nTracer was written as a plug-in for the open source image processing software ImageJ. The software, instructional documentation, tutorial videos, sample image and sample t ...[more]
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