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Alkaloid Enantiomers from the Roots of Isatis indigotica.

ABSTRACT: Five pairs of alkaloid enantiomers (1a/1b-5a/5b) were obtained from Isatis indigotica (I. indigotica) roots. Among them, 1a/1b, 2a/2b and 3a/3b were determined as three pairs of new alkaloid enantiomers. Their structures were elucidated by physicochemical properties and spectroscopic methods. The absolute configurations were deduced by comparison of their experimental circular dichroism (CD) and calculated electronic circular dichroism (ECD) spectra, as well as by single-crystal X-ray crystallography using anomalous scattering of Cu K? radiation. Alkaloids 1a and 1b possess an unpresented carbon skeleton and their putative biosynthetic pathways are discussed. Moreover, all of the alkaloids were tested for their nitric oxide (NO) inhibitory effects in RAW 264.7 cells, and 4a and 4b showed inhibitory effects with IC₅₀ values of 76.97 ?M and 65.88 ?M, respectively.

SUBMITTER: Zhang D

PROVIDER: S-EPMC6749297 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Alkaloid Enantiomers from the Roots of Isatis indigotica.

Zhang Dongdong D Shi Yanhong Y Xu Rui R Du Kang K Guo Fujiang F Chen Kaixian K Li Yiming Y Wang Rui R

Molecules (Basel, Switzerland) 20190829 17

Five pairs of alkaloid enantiomers (1a/1b-5a/5b) were obtained from Isatis indigotica (I. indigotica) roots. Among them, 1a/1b, 2a/2b and 3a/3b were determined as three pairs of new alkaloid enantiomers. Their structures were elucidated by physicochemical properties and spectroscopic methods. The absolute configurations were deduced by comparison of their experimental circular dichroism (CD) and calculated electronic circular dichroism (ECD) spectra, as ...[more]

PMID: 31470525

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