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Catalpol ameliorates LPS-induced endometritis by inhibiting inflammation and TLR4/NF-?B signaling.

ABSTRACT: Catalpol is the main active ingredient of an extract from Radix rehmanniae, which in a previous study showed a protective effect against various types of tissue injury. However, a protective effect of catalpol on uterine inflammation has not been reported. In this study, to investigate the protective mechanism of catalpol on lipopolysaccharide (LPS)-induced bovine endometrial epithelial cells (bEECs) and mouse endometritis, in vitro and in vivo inflammation models were established. The Toll-like receptor 4 (TLR4)/nuclear factor-?B (NF-?B) signaling pathway and its downstream inflammatory factors were detected by enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qRT-PCR), western blot (WB), and immunofluorescence techniques. The results from ELISA and qRT-PCR showed that catalpol dose-dependently reduced the expression of pro-inflammatory cytokines such as tumor necrosis factor ? (TNF-?), interleukin (IL)-1?, and IL-6, and chemokines such as C-X-C motif chemokine ligand 8 (CXCL8) and CXCL5, both in bEECs and in uterine tissue. From the experimental results of WB, qRT-PCR, and immuno?uorescence, the expression of TLR4 and the phosphorylation of NF-?B p65 were markedly inhibited by catalpol compared with the LPS group. The inflammatory damage to the mouse uterus caused by LPS was greatly reduced and was accompanied by a decline in myeloperoxidase (MPO) activity. The results of this study suggest that catalpol can exert an anti-inflammatory impact on LPS-induced bEECs and mouse endometritis by inhibiting inflammation and activation of the TLR4/NF-?B signaling pathway.

SUBMITTER: Zhang H

PROVIDER: S-EPMC6751487 | biostudies-literature | 2019 Oct.

REPOSITORIES: biostudies-literature

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Catalpol ameliorates LPS-induced endometritis by inhibiting inflammation and TLR4/NF-κB signaling.

Zhang Hua H Wu Zhi-Min ZM Yang Ya-Ping YP Shaukat Aftab A Yang Jing J Guo Ying-Fang YF Zhang Tao T Zhu Xin-Ying XY Qiu Jin-Xia JX Deng Gan-Zhen GZ Shi Dong-Mei DM

Journal of Zhejiang University. Science. B 20191001 10

Catalpol is the main active ingredient of an extract from Radix rehmanniae, which in a previous study showed a protective effect against various types of tissue injury. However, a protective effect of catalpol on uterine inflammation has not been reported. In this study, to investigate the protective mechanism of catalpol on lipopolysaccharide (LPS)-induced bovine endometrial epithelial cells (bEECs) and mouse endometritis, in vitro and in vivo inflammation models were established. The Toll-like ...[more]

PMID: 31489801

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Catalpol ameliorates LPS-induced endometritis by inhibiting inflammation and TLR4/NF-?B signaling.

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Catalpol ameliorates LPS-induced endometritis by inhibiting inflammation and TLR4/NF-κB signaling.

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