Dataset Information

Photopheresis efficacy in the treatment of rheumatoid arthritis: a pre-clinical proof of concept.

ABSTRACT:

Background

Despite major advances in rheumatoid arthritis outcome, not all patients achieve remission, and there is still an unmet need for new therapeutic approaches. This study aimed at evaluating in a pre-clinical murine model the efficacy of extracorporeal photopheresis (ECP) in the treatment of rheumatoid arthritis, and to provide a relevant study model for dissecting ECP mechanism of action in autoimmune diseases.

Methods

DBA/1 mice were immunized by subcutaneous injection of bovine collagen type II, in order to initiate the development of collagen-induced arthritis (CIA). Arthritic mice received 3 ECP treatments every other day, with psoralen?+?UVA-treated (PUVA) spleen cells obtained from arthritic mice. Arthritis score was measured, and immune cell subsets were monitored.

Results

ECP-treated mice recovered from arthritis as evidenced by a decreasing arthritic score over time. Significant decrease in the frequency of Th17 cells in the spleen of treated mice was observed. Interestingly, while PUVA-treated spleen cells from healthy mouse had no effect, PUVA-treated arthritic mouse derived-spleen cells were able to induce control of arthritis development.

Conclusions

Our results demonstrate that ECP can control arthritis in CIA-mice, and clarifies ECP mechanisms of action, showing ECP efficacy and Th17 decrease only when arthritogenic T cells are contained within the treated sample. These data represent a pre-clinical proof of concept supporting the use of ECP in the treatment of RA in Human.

SUBMITTER: Coppard C

PROVIDER: S-EPMC6751641 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Publications

Photopheresis efficacy in the treatment of rheumatoid arthritis: a pre-clinical proof of concept.

Coppard Céline C Bonnefoy Francis F Hannani Dalil D Gabert Françoise F Manches Olivier O Plumas Joel J Perruche Sylvain S Chaperot Laurence L

Journal of translational medicine 20190918 1

<h4>Background</h4>Despite major advances in rheumatoid arthritis outcome, not all patients achieve remission, and there is still an unmet need for new therapeutic approaches. This study aimed at evaluating in a pre-clinical murine model the efficacy of extracorporeal photopheresis (ECP) in the treatment of rheumatoid arthritis, and to provide a relevant study model for dissecting ECP mechanism of action in autoimmune diseases.<h4>Methods</h4>DBA/1 mice were immunized by subcutaneous injection o ...[more]

PMID: 31533744

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Dataset Information

Photopheresis efficacy in the treatment of rheumatoid arthritis: a pre-clinical proof of concept.

Background

Methods

Results

Conclusions

Publications

Photopheresis efficacy in the treatment of rheumatoid arthritis: a pre-clinical proof of concept.

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