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Prebiotic Phosphorylation of Uridine using Diamidophosphate in Aerosols.

ABSTRACT: One of the most challenging fundamental problems in establishing prebiotically plausible routes for phosphorylation reactions using phosphate is that they are thermodynamically unfavorable in aqueous conditions. Diamidophosphate (DAP), a potentially prebiotically relevant compound, was shown to phosphorylate nucleosides in aqueous medium, albeit at a very slow rate (days/weeks). Here, we demonstrate that performing these reactions within an aerosol environment, a suitable model for the early Earth ocean-air interface, yields higher reaction rates when compared to bulk solution, thus overcoming these rate limitations. As a proof-of-concept, we demonstrate the effective conversion (~6.5-10%) of uridine to uridine-2',3'-cyclophosphate in less than 1?h. These results suggest that aerosol environments are a possible scenario in which prebiotic phosphorylation could have occurred despite unfavorable rates in bulk solution.

SUBMITTER: Castaneda AD

PROVIDER: S-EPMC6753121 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Prebiotic Phosphorylation of Uridine using Diamidophosphate in Aerosols.

Castañeda A D AD Li Z Z Joo T T Benham K K Burcar B T BT Krishnamurthy R R Liotta C L CL Ng N L NL Orlando T M TM

Scientific reports 20190919 1

One of the most challenging fundamental problems in establishing prebiotically plausible routes for phosphorylation reactions using phosphate is that they are thermodynamically unfavorable in aqueous conditions. Diamidophosphate (DAP), a potentially prebiotically relevant compound, was shown to phosphorylate nucleosides in aqueous medium, albeit at a very slow rate (days/weeks). Here, we demonstrate that performing these reactions within an aerosol environment, a suitable model for the early Ear ...[more]

PMID: 31537885

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