Ontology highlight
ABSTRACT:
SUBMITTER: Kendall C
PROVIDER: S-EPMC6753490 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Kendall Catherine C Khalid Henna H Müller Marietta M Banda Dominic H DH Kohl Alain A Merits Andres A Stonehouse Nicola J NJ Tuplin Andrew A
Nucleic acids research 20190901 17
Chikungunya virus (CHIKV) is a re-emerging, pathogenic Alphavirus transmitted to humans by Aedes spp. mosquitoes. We have mapped the RNA structure of the 5' region of the CHIKV genome using selective 2'-hydroxyl acylation analysed by primer extension (SHAPE) to investigate intramolecular base-pairing at single-nucleotide resolution. Taking a structure-led reverse genetic approach, in both infectious virus and sub-genomic replicon systems, we identified six RNA replication elements essential to e ...[more]