Ontology highlight
ABSTRACT:
SUBMITTER: Lin W
PROVIDER: S-EPMC6754165 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Lin Wei W Wang Dongping D Meng Yunlong Y Chen Shih-Chi SC
PloS one 20190920 9
In this paper, we present a new multi-focus microscope (MFM) system based on a phase mask and HiLo algorithm, achieving high-speed (20 volumes per second), high-resolution, low-noise 3-D fluorescent imaging. During imaging, the emissions from the specimen at nine different depths are simultaneously modulated and focused to different regions on a single CCD chip, i.e., the CCD chip is subdivided into nine regions to record images from the different selected depths. Next, HiLo algorithm is applied ...[more]