Light-based control of metabolic flux through assembly of synthetic organelles.
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ABSTRACT:
SUBMITTER: Zhao EM
PROVIDER: S-EPMC6755918 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Publications
Light-based control of metabolic flux through assembly of synthetic organelles.
Zhao Evan M EM Suek Nathan N Wilson Maxwell Z MZ Dine Elliot E Pannucci Nicole L NL Gitai Zemer Z Avalos José L JL Toettcher Jared E JE
Nature chemical biology 20190513 6
To maximize a desired product, metabolic engineers typically express enzymes to high, constant levels. Yet, permanent pathway activation can have undesirable consequences including competition with essential pathways and accumulation of toxic intermediates. Faced with similar challenges, natural metabolic systems compartmentalize enzymes into organelles or post-translationally induce activity under certain conditions. Here we report that optogenetic control can be used to extend compartmentaliza ...[more]
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