Dataset Information

The microbial community in filamentous bulking sludge with the ultra-low sludge loading and long sludge retention time in oxidation ditch.

ABSTRACT: Sludge bulking is a major problem that restricts the development of the activated sludge process. The microbial community responsible for sludge bulking varies depending on water quality and operational conditions. This study analysed the microbial community of bulking sludge in oxidation ditch with ultra-low sludge loading and long sludge retention time using high-throughput sequencing. The study found that the relative abundance of bacterial genus Saprospiraceae_norank was the highest in bulking sludge, reaching 13.39-28.83%, followed by Comamonadaceae_unclassified, Ardenticatenia_norank and Tetrasphaera, with the relative abundance of 4.59-11.08%, 0.52-16.60% and 0.17-8.92% respectively. In contrast, the relative abundance of bacteria that easily caused sludge bulking including Microthrix (0.54-2.47%), Trichococcus (0.32-1.71%), Gordonia (0.14-1.28%), and Thiothrix (0.01-0.06%) were relatively low. Saprospiraceae_norank was predominant and induced sludge bulking in oxidation ditch. The relative abundance of fungal genus Trichosporon was the highest in bulking sludge, reaching 16.95-24.98%, while other fungal genera were Saccharomycetales_unclassified (5.59-14.55%), Ascomycota_norank (1.45-13.51%), Galactomyces (5.23-11.23%), and Debaryomyces (7.69-9.42%), whereas Trichosporon was the dominant fungal genus in bulking sludge. This study reported that excessive Saprospiraceae_norank can induce sludge bulking for the first time, which provides important knowledge to control sludge bulking.

SUBMITTER: Zhang M

PROVIDER: S-EPMC6757048 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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The microbial community in filamentous bulking sludge with the ultra-low sludge loading and long sludge retention time in oxidation ditch.

Zhang Meng M Yao Junqin J Wang Xiyuan X Hong Ying Y Chen Yinguang Y

Scientific reports 20190923 1

Sludge bulking is a major problem that restricts the development of the activated sludge process. The microbial community responsible for sludge bulking varies depending on water quality and operational conditions. This study analysed the microbial community of bulking sludge in oxidation ditch with ultra-low sludge loading and long sludge retention time using high-throughput sequencing. The study found that the relative abundance of bacterial genus Saprospiraceae_norank was the highest in bulki ...[more]

PMID: 31548550

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Project description:Wastewater treatment plants (WWTPs) are host to diverse microbial communities and receive a constant influx of microbes from influent wastewater. However, the impact of immigrants on the structure and activities of the activated sludge (AS) microbial community remains unclear. To gain insight on this phenomenon known as perpetual community coalescence, the current study utilized controlled manipulative experiments that decoupled the influent wastewater composition from the microbial populations to reveal the fundamental mechanisms involved in immigration between sewers and AS-WWTP. The immigration dynamics of heterotrophs were analyzed by harvesting wastewater biomass solids from three different sewer systems and adding to synthetic wastewater. Immigrating influent populations were observed to contribute up to 14% of the sequencing reads in the AS. By modeling the net growth rate of taxa, it was revealed that immigrants primarily exhibited low or negative net growth rates. By developing a protocol to reproducibly grow AS-WWTP communities in the lab, we have laid down the foundational principles for the testing of operational factors creating community variations with low noise and appropriate replication. Understanding the processes that drive microbial community diversity and assembly is a key question in microbial ecology. In the future, this knowledge can be used to manipulate the structure of microbial communities and improve system performance in WWTPs.IMPORTANCEIn biological wastewater treatment processes, the microbial community composition is essential in the performance and stability of the system. This study developed a reproducible protocol to investigate the impact of influent immigration (or perpetual coalescence of the sewer and activated sludge communities) with appropriate reproducibility and controls, allowing intrinsic definitions of core and immigrant populations to be established. The method developed herein will allow sequential manipulative experiments to be performed to test specific hypothesis and optimize wastewater treatment processes to meet new treatment goals.

Dataset Information

The microbial community in filamentous bulking sludge with the ultra-low sludge loading and long sludge retention time in oxidation ditch.

Publications

The microbial community in filamentous bulking sludge with the ultra-low sludge loading and long sludge retention time in oxidation ditch.

Similar Datasets

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