Evaluation of immuno-modulating effect of recombinant heat shock protein 40 of Brucella abortus in mice.
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ABSTRACT:
SUBMITTER: Minhas P
PROVIDER: S-EPMC6757077 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
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Evaluation of immuno-modulating effect of recombinant heat shock protein 40 of <i>Brucella abortus</i> in mice.
Minhas Priyanka P Sunil Kumar B V BV Verma Ramneek R
3 Biotech 20190923 10
The present study was aimed to evaluate the immuno-modulatory effect of <i>Brucella</i>-specific recombinant HSP40 (rDnaJ) when co-immunized with <i>Brucella</i> rOmp22 in mice. For this, <i>dnaJ</i> of <i>Brucella abortus</i> was cloned, expressed in <i>E. coli,</i> and purified to homogeneity using Ni-NTA agarose columns. Three groups of mice (<i>n</i> = 6 in each group) were used in the study. The control group was immunized with rOmp22 alone, while group 1 mice were injected subcutaneously w ...[more]
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