Dataset Information

Central Treatment of Ketone Body in Rainbow Trout Alters Liver Metabolism Without Apparently Altering the Regulation of Food Intake.

ABSTRACT: We hypothesize that the presence in fish brain of a ketone body (KB) like ?-hydroxybutyrate (BHB) alters energy homeostasis through effects on food intake and peripheral energy metabolism. Using rainbow trout (Oncorhynchus mykiss) as a model, we intracerebroventricularly (ICV) administered 1 ?l 100 g-1 body mass of saline solution alone (control) or containing 0.5 ?mol of BHB. In a fist set of experiments, BHB did not affect food intake 6 and 24 h after treatment. In a second set of experiments, we evaluated 6 h after ICV BHB treatment changes in parameters putatively related to food intake control in brain areas (hypothalamus and hindbrain) involved in nutrient sensing and changes in energy metabolism in liver. The absence of changes in food intake might relate to the absence of major changes in the cascade of events from the detection of KB through ketone-sensing mechanisms, changes in transcription factors, and changes in the mRNA abundance of neuropeptides regulating food intake. This response is different than that of mammals. In contrast, central administration of BHB induced changes in liver energy metabolism suggesting a decreased use of glucose and probably an enhanced use of amino acid and lipid. These responses in liver are different to those of mammals under similar treatments but comparable to those occurring in fish under food deprivation conditions.

SUBMITTER: Comesana S

PROVIDER: S-EPMC6759561 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

Central Treatment of Ketone Body in Rainbow Trout Alters Liver Metabolism Without Apparently Altering the Regulation of Food Intake.

Comesaña Sara S Velasco Cristina C Conde-Sieira Marta M Otero-Rodiño Cristina C Míguez Jesús M JM Soengas José L JL

Frontiers in physiology 20190918

We hypothesize that the presence in fish brain of a ketone body (KB) like β-hydroxybutyrate (BHB) alters energy homeostasis through effects on food intake and peripheral energy metabolism. Using rainbow trout (<i>Oncorhynchus mykiss</i>) as a model, we intracerebroventricularly (ICV) administered 1 μl 100 g<sup>-1</sup> body mass of saline solution alone (control) or containing 0.5 μmol of BHB. In a fist set of experiments, BHB did not affect food intake 6 and 24 h after treatment. In a second s ...[more]

PMID: 31620022

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Project description:To continue gathering knowledge on the central regulation of food intake in response to amino acids in teleost fish, using as a model rainbow trout (Oncorhynchus mykiss), we evaluated in a first experiment the feeding attractiveness of L-leucine, L-valine, and L-proline offered as an agar gel matrix. In a second experiment, we assessed the effect of intraperitoneal (IP) treatment with the same amino acids on food intake. In a third experiment, we carried out a similar IP administration of amino acids to evaluate the response of amino acid sensing mechanisms in the hypothalamus and telencephalon. Results are discussed in conjunction with an earlier study where leucine and valine were administered intracerebroventricularly (ICV). The attractiveness of amino acids does not appear to relate to their effects on food intake, at least when administrated by-passing ingestion and luminal absorption, since two attractive amino acids resulted in an anorexigenic (Leu) or no effects (Pro) on food intake while a non-attractive amino acid (Val) induced anorexigenic (IP treatment) or orexigenic (ICV treatment) responses. The effects of Leu on food intake might relate to the expression of hypothalamic neuropeptides and result from the direct activation of amino acid sensing systems. In contrast, while valine had few effects on hypothalamic amino acid sensing systems after ICV treatment, a significant amount of parameters become affected by IP treatment suggesting that the effect of Val after IP treatment is indirect. Proline had no relevant effects on amino acid sensing systems, neuropeptide expression, and food intake, which suggest that this amino acid might not have a relevant role in the homeostatic regulation of food intake through hypothalamic mechanisms. In telencephalon, the same amino acid sensing systems operating in hypothalamus appear to be present and respond to Leu and Val, but it is still unclear how they might relate to the control of food intake.

Dataset Information

Central Treatment of Ketone Body in Rainbow Trout Alters Liver Metabolism Without Apparently Altering the Regulation of Food Intake.

Publications

Central Treatment of Ketone Body in Rainbow Trout Alters Liver Metabolism Without Apparently Altering the Regulation of Food Intake.

Similar Datasets

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