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Age- and sex-dependent role of osteocytic pannexin1 on bone and muscle mass and strength.

ABSTRACT: Pannexins (Panxs), glycoproteins that oligomerize to form hemichannels on the cell membrane, are topologically similar to connexins, but do not form cell-to-cell gap junction channels. There are 3 members of the family, 1-3, with Panx1 being the most abundant. All Panxs are expressed in bone, but their role in bone cell biology is not completely understood. We now report that osteocytic Panx1 deletion (Panx1?ot) alters bone mass and strength in female mice. Bone mineral density after reaching skeletal maturity is higher in female Panx1?ot mice than in control Panx1fl/fl mice. Further, osteocytic Panx1 deletion partially prevented aging effects on cortical bone structure and mechanical properties. Young 4-month-old female Panx1?ot mice exhibited increased lean body mass, even though pannexin levels in skeletal muscle were not affected; whereas no difference in lean body mass was detected in male mice. Furthermore, female Panx1-deficient mice exhibited increased muscle mass without changes in strength, whereas Panx1?ot males showed unchanged muscle mass and decreased in vivo maximum plantarflexion torque, indicating reduced muscle strength. Our results suggest that osteocytic Panx1 deletion increases bone mass in young and old female mice and muscle mass in young female mice, but has deleterious effects on muscle strength only in males.

SUBMITTER: Aguilar-Perez A

PROVIDER: S-EPMC6761284 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Age- and sex-dependent role of osteocytic pannexin1 on bone and muscle mass and strength.

Aguilar-Perez Alexandra A Pacheco-Costa Rafael R Atkinson Emily G EG Deosthale Padmini P Davis Hannah M HM Essex Alyson L AL Dilley Julian E JE Gomez Leland L Rupert Joseph E JE Zimmers Teresa A TA Thompson Roger J RJ Allen Matthew R MR Plotkin Lilian I LI

Scientific reports 20190925 1

Pannexins (Panxs), glycoproteins that oligomerize to form hemichannels on the cell membrane, are topologically similar to connexins, but do not form cell-to-cell gap junction channels. There are 3 members of the family, 1-3, with Panx1 being the most abundant. All Panxs are expressed in bone, but their role in bone cell biology is not completely understood. We now report that osteocytic Panx1 deletion (Panx1<sup>Δot</sup>) alters bone mass and strength in female mice. Bone mineral density after ...[more]

PMID: 31554905

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Dataset Information

Age- and sex-dependent role of osteocytic pannexin1 on bone and muscle mass and strength.

Publications

Age- and sex-dependent role of osteocytic pannexin1 on bone and muscle mass and strength.

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