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FgPex3, a Peroxisome Biogenesis Factor, Is Involved in Regulating Vegetative Growth, Conidiation, Sexual Development, and Virulence in Fusarium graminearum.


ABSTRACT: Peroxisomes are involved in a wide range of important cellular functions. Here, the role of the peroxisomal membrane protein PEX3 in the plant-pathogen and mycotoxin producer Fusarium graminearum was studied using knock-out and complemented strains. To fluorescently label peroxisomes' punctate structures, GFP and RFP fusions with the PTS1 and PTS2 localization signal were transformed into the wild type PH-1 and ?FgPex3 knock-out strains. The GFP and RFP transformants in the ?FgPex3 background showed a diffuse fluorescence pattern across the cytoplasm suggesting the absence of mature peroxisomes. The ?FgPex3 strain showed a minor, non-significant reduction in growth on various sugar carbon sources. In contrast, deletion of FgPex3 affected fatty acid ?-oxidation in F. graminearum and significantly reduced the utilization of fatty acids. Furthermore, the ?FgPex3 mutant was sensitive to osmotic stressors as well as to cell wall-damaging agents. Reactive oxygen species (ROS) levels in the mutant had increased significantly, which may be linked to the reduced longevity of cultured strains. The mutant also showed reduced production of conidiospores, while sexual reproduction was completely impaired. The pathogenicity of ?FgPex3, especially during the process of systemic infection, was strongly reduced on both tomato and on wheat, while to production of deoxynivalenol (DON), an important factor for virulence, appeared to be unaffected.

SUBMITTER: Kong X 

PROVIDER: S-EPMC6764106 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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<i>FgPex3</i>, a Peroxisome Biogenesis Factor, Is Involved in Regulating Vegetative Growth, Conidiation, Sexual Development, and Virulence in <i>Fusarium graminearum</i>.

Kong Xiangjiu X   Zhang Hao H   Wang Xiaoliang X   van der Lee Theo T   Waalwijk Cees C   van Diepeningen Anne A   Brankovics Balazs B   Xu Jin J   Xu Jingsheng J   Chen Wanquan W   Feng Jie J  

Frontiers in microbiology 20190920


Peroxisomes are involved in a wide range of important cellular functions. Here, the role of the peroxisomal membrane protein PEX3 in the plant-pathogen and mycotoxin producer <i>Fusarium graminearum</i> was studied using knock-out and complemented strains. To fluorescently label peroxisomes' punctate structures, GFP and RFP fusions with the PTS1 and PTS2 localization signal were transformed into the wild type PH-1 and Δ<i>FgPex3</i> knock-out strains. The GFP and RFP transformants in the Δ<i>FgP  ...[more]

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