Dataset Information

ViralPlaque: a Fiji macro for automated assessment of viral plaque statistics.

ABSTRACT: Plaque assay has been used for a long time to determine infectious titers and characterize prokaryotic and eukaryotic viruses forming plaques. Indeed, plaque morphology and dimensions can provide information regarding the replication kinetics and the virulence of a particular virus. In this work, we present ViralPlaque, a fast, open-source and versatile ImageJ macro for the automated determination of viral plaque dimensions from digital images. Also, a machine learning plugin is integrated in the analysis algorithm for adaptation of ViralPlaque to the user's needs and experimental conditions. A high correlation between manual and automated measurements of plaque dimensions was demonstrated. This macro will facilitate reliable and reproducible characterization of cytolytic viruses with an increased processing speed.

SUBMITTER: Cacciabue M

PROVIDER: S-EPMC6764358 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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ViralPlaque: a Fiji macro for automated assessment of viral plaque statistics.

Cacciabue Marco M Currá Anabella A Gismondi Maria I MI

PeerJ 20190924

Plaque assay has been used for a long time to determine infectious titers and characterize prokaryotic and eukaryotic viruses forming plaques. Indeed, plaque morphology and dimensions can provide information regarding the replication kinetics and the virulence of a particular virus. In this work, we present ViralPlaque, a fast, open-source and versatile ImageJ macro for the automated determination of viral plaque dimensions from digital images. Also, a machine learning plugin is integrated in th ...[more]

PMID: 31579606

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Dataset Information

ViralPlaque: a Fiji macro for automated assessment of viral plaque statistics.

Publications

ViralPlaque: a Fiji macro for automated assessment of viral plaque statistics.

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