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Gold Nanoparticle-Functionalized Reverse Thermal Gel for Tissue Engineering Applications.

ABSTRACT: Utilizing polymers in cardiac tissue engineering holds promise for restoring function to the heart following myocardial infarction, which is associated with grave morbidity and mortality. To properly mimic native cardiac tissue, materials must not only support cardiac cell growth but also have inherent conductive properties. Here, we present an injectable reverse thermal gel (RTG)-based cardiac cell scaffold system that is both biocompatible and conductive. Following the synthesis of a highly functionalizable, biomimetic RTG backbone, gold nanoparticles (AuNPs) were chemically conjugated to the backbone to enhance the system's conductivity. The resulting RTG-AuNP hydrogel supported targeted survival of neonatal rat ventricular myocytes (NRVMs) for up to 21 days when cocultured with cardiac fibroblasts, leading to an increase in connexin 43 (Cx43) relative to control cultures (NRVMs cultured on traditional gelatin-coated dishes and RTG hydrogel without AuNPs). This biomimetic and conductive RTG-AuNP hydrogel holds promise for future cardiac tissue engineering applications.

SUBMITTER: Pena B

PROVIDER: S-EPMC6764451 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Gold Nanoparticle-Functionalized Reverse Thermal Gel for Tissue Engineering Applications.

Peña Brisa B Maldonado Marcos M Bonham Andrew J AJ Aguado Brian A BA Dominguez-Alfaro Antonio A Laughter Melissa M Rowland Teisha J TJ Bardill James J Farnsworth Nikki L NL Alegret Ramon Nuria N Taylor Matthew R G MRG Anseth Kristi S KS Prato Maurizio M Shandas Robin R McKinsey Timothy A TA Park Daewon D Mestroni Luisa L

ACS applied materials & interfaces 20190507 20

Utilizing polymers in cardiac tissue engineering holds promise for restoring function to the heart following myocardial infarction, which is associated with grave morbidity and mortality. To properly mimic native cardiac tissue, materials must not only support cardiac cell growth but also have inherent conductive properties. Here, we present an injectable reverse thermal gel (RTG)-based cardiac cell scaffold system that is both biocompatible and conductive. Following the synthesis of a highly fu ...[more]

PMID: 31021594

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Gold Nanoparticle-Functionalized Reverse Thermal Gel for Tissue Engineering Applications.

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Gold Nanoparticle-Functionalized Reverse Thermal Gel for Tissue Engineering Applications.

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