Dataset Information

Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America.

ABSTRACT: Premise:A novel set of nuclear microsatellite markers was developed and characterized for Campomanesia adamantium (Myrtaceae) and tested for cross-amplification in the related species C. sessiliflora. Methods and Results:Forty-one primer pairs were designed for simple sequence repeat loci, of which 36 successfully amplified and were polymorphic. The number of alleles ranged from two to 14, with an average of 8.14 alleles per locus. Additionally, cross-amplification was tested in C. sessiliflora; more than 55.5% of the microsatellite loci amplified, confirming the use of these microsatellite markers in a related species. Conclusions:We developed a set of microsatellite markers that will be useful for future studies of genetic diversity and population structure of C. adamantium and a closely related species, which will aid in future conservation efforts.

SUBMITTER: Crispim BDA

PROVIDER: S-EPMC6764490 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Publications

Development and characterization of microsatellite markers in <i>Campomanesia adamantium</i>, a native plant of the Cerrado ecoregions of South America.

Crispim Bruno do Amaral BDA Déo Thamiris Gatti TG Fernandes Juliana Dos Santos JDS de Vasconcelos Adrielle Ayumi AA Vieira Maria do Carmo MDC Carnevali Thiago de Oliveira TO Bajay Miklos Maximiliano MM Zucchi Maria Imaculada MI Barufatti Alexeia A

Applications in plant sciences 20190922 9

<h4>Premise</h4>A novel set of nuclear microsatellite markers was developed and characterized for <i>Campomanesia adamantium</i> (Myrtaceae) and tested for cross-amplification in the related species <i>C. sessiliflora</i>.<h4>Methods and results</h4>Forty-one primer pairs were designed for simple sequence repeat loci, of which 36 successfully amplified and were polymorphic. The number of alleles ranged from two to 14, with an average of 8.14 alleles per locus. Additionally, cross-amplification w ...[more]

PMID: 31572628

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Project description:The 361 species of hummingbirds that occur from Alaska to Patagonia pollinate ~7,000 plant species with flowers morphologically adapted to them. To better understand this asymmetric diversity build-up, this study analyzes the origin of hummingbird/plant mutualisms in North America and temperate South America, based on new compilations of the 184 hummingbird-adapted species in North America, the 56 in temperate South America, and complete species-level phylogenies for the relevant hummingbirds in both regions, namely five in temperate South America and eight in North America. Because both floras are relatively well sampled phylogenetically, crown or stem ages of many representative clades could be inferred. The hummingbird chronogram was calibrated once with fossils, once with substitutions rates, while plant chronograms were taken from the literature or in 13 cases newly generated.The 184 North American hummingbird-adapted species belong to ca. 70 lineages for 19 of which (comprising 54 species) we inferred divergence times. The 56 temperate South American hummingbird-adapted species belong to ca. 35 lineages, for 17 of which (comprising 25 species) we inferred divergence times. The oldest hummingbirds and hummingbird-adapted plant lineages in the South American assemblage date to 16-17 my, those in the North American assemblage to 6-7 my. Few hummingbird-pollinated clades in either system have >4 species.The asymmetric diversity build-up between hummingbirds and the plants dependent on them appears to arise not from rapid speciation within hummingbird-pollinated clades, but instead from a gradual and continuing process in which independent plant species switch from insect to bird pollination. Diversification within hummingbird-pollinated clades in the temperate regions of the Americas appears mainly due to habitat specialization and allopatric speciation, not bird pollination per se. Interaction tanglegrams, even if incomplete, indicate a lack of tight coevolution as perhaps expected for temperate-region mutualisms involving nectar-feeding vertebrates.

Dataset Information

Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America.

Publications

Development and characterization of microsatellite markers in <i>Campomanesia adamantium</i>, a native plant of the Cerrado ecoregions of South America.

Similar Datasets

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